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We again detected a higher amount of biotinylated nephrin in PKCα−/− podocytes indicating a higher rate of endocytosis in the wildtype cells (Fig. 4E).

Although we again detected a functional redundancy of both Sharp genes in this paradigm, Sharp-2 loss-of-function cannot be fully compensated for by only one Sharp-1 wild-type allele.

Using the flowering-time-matched plants (experiment 4, Table  1), we again detected significant variation in all traits examined.

We again detected spikes in intensity upon addition of Nup153FG indicating the formation of large complexes (new Figure 6D).

We again detected a statistically significant number of genes DE between preM and postM in ER+ but not in ER– tumors (data not shown).

We again detected the presence of LC8 in cilia, in addition to a strong signal at the base of cilia (Fig. 7B); puncta within the nucleus were also evident.

When we combine our results for DNA-mediated and RNA-mediated duplicates with 50% peptide sequence alignments/40% amino acid percent identity, we again detect no excess involving the Z chromosome (table 3D: χ = 0.71, df = 1, P = 0.396; supplementary table 7, Supplementary Material online).

Moreover, we have again detected a critical strain threshold in chondrocytes at ∼30% strain in all treatments.

Continuous monitoring of human rotavirus strains circulating in local communities will be important to determine if we are again detecting the early stages of the global emergence of a novel genetic lineage of an "old" rotavirus serotype.

We note, however, that after cidofovir therapy was stopped, HHV-6 DNA was again detected in the CSF, concurrent with an increase in neurologic symptoms.

We also repeated the association tests in the subset of individuals, carriers of the HLA-Bw4 epitope, and again detected no significant association (Table 2).