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We additionally checked the influence using a case where the linear expansion coefficient had an uncertainty of 5.0 × 10−7/°C, where the temperature difference was 10 °C and where the maximum relative height from benchmark BM34 was approximately 350 m.
Whenever weighted regression was used we additionally checked that the non-parametric Spearman rank test yielded the same conclusion.
We additionally checked the probability of reaching the G1 stationary state, starting only from those initial conditions, when "Start" is active on the first time step.
We additionally checked these Transcript Active Regions (TARs) for open reading frames.
Thus, we additionally checked our specific gene sets of interest ("regulated", "targets", "regulated targets") for the presence of an expression bias in general (Author response image 5).
As OLP did not pass the Shapiro-Wilk test, we additionally checked the q-q plot, which did not show marked deviation from linearity.
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As this imposes the assumption that the trend divergence is linear, we additionally check alternative more flexible specifications of such trends by the use of quadratic and cubic polynomials, i.e., begin{array}rcl@ Psi_{mathrm{treatment,group,t}}=T_{t}*mathrm{treatment,group}_{i}*psi_{1}+T_{t}^{2}*mathrm{treatment,group}_{i}*psi_{2} end{array}.
We thus additionally checked the significance of Pearson R to test the strength of correlation of the superior or inferior angle with each of those sectors.
We also additionally checked the expression specificity of the selected marker genes in the Allen Brain Atlas and discarded GAD1 (Purkinje cell marker) because it showed strong expression in Golgi cells (in addition to Purkinje cells, see also Schilling et al. [ 40]).
Temperature control was achieved using a Vötsch climatic chamber with the temperature being additionally checked by a thermocouple.
The obtained information have been interpreted on the base of the surface morphology, additionally checked by the scanning electron microscope (SEM).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com