We meet Jonathan, who is in his 20's and the son of a businessman we have previously encountered, when he bursts into a bar and frantically engages a strange woman in conversation because, we subsequently find out, a fortuneteller has predicted he will meet his soul mate by midnight.
As we had previously encountered in sequencing the entire genome of TPMV (J.-W. Song, R. Yanagihara, unpub. data), the divergent genome of RPLV presented challenges in designing suitable primers for RT-PCR.
Ten L1 insertions detected only at a 5′ L1-genome junction were also tested, although as we have previously encountered (Baillie et al., 2011 ), PCR amplification of the corresponding 3′ L1-genome junction was extremely challenging, and none of these examples were confirmed (Table S2 ).
"It's unlike anything I've previously encountered".
But some of the puzzles are more difficult than any I've previously encountered in the series.
Given that PBMC are capable of trafficking to the inner ear during inflammation [2], we anticipated that immunocytes that had previously encountered a cochlear antigen would respond differently to cochlear proteins compared with those that had not been previously exposed.
We started with an icebreaker to discuss common difficulties that teachers had previously encountered with student comments or confusion surrounding evolutionary topics.
However, if the number of var genes that a host has previously encountered relates to the degree of protection against clinical disease, we would expect more severe symptoms to occur at younger ages under high transmission settings, and in contrast, more homogeneous malaria incidents are likely to occur over a broader range of age-classes when transmission is lowered.
Hillary and Bill Clinton have previously encountered marital and legal problems.
Unlike Mr. Daniels, Ms. Harden had previously encountered Mr. Gandolfini, whom she almost cooingly described as soulful.
