Kinetic modeling represents a more complicated but preferable way of quantification.
Because luciferase reporter can provide a cost-effective way of quantification, together this dual reporter system offers a powerful tool superior to any single reporter system.
It seems to be a good way for quantification of the maps that look similarly.
A more detailed study is currently underway, as this might open the way for quantification of the probe ratios in individual spot, especially when combined with more rigorous global analysis of the lifetime images [ 19].
This way of flow quantification seems, therefore, potentially applicable to the analysis of OPS imaging in many more microvascular structures not yet described in the literature.
The ability of real-time Q-PCR to detect the fluorescent signal from degraded sequence specific TaqMan probe at the very beginning period of exponential stage offered an accurate way of DNA quantification.
Novel pattern recognition algorithms and digital fluorescence have paved the way for quantification and automation of autoAb testing through IIF interpretation systems [33, 34, 35, 36].
Other label free ways of large scale protein quantification by MS make use of correlations between the number of actually identified tryptic peptides per protein and the theoretical number of tryptic peptides [ 16], or the molecular weight of the proteins [ 17].
Procedure solvent blank samples representing every seventh sample were analyzed in the same way as the serum extracts Limits of quantification (LO Qs were defined in direct relation to the amount of interference of PBDEs in the blank samples.
