Exact(33)
In all experiments, images of cells (1024×1024 pixels) were visualized using the same confocal microscope settings (i.e. sequential scans with wavelengths set as follows: blue, 358 461; green, 488 510; red, 594 615), using a 63× Apo-oil immersion objective (NA=1.4) and 60-μm aperture, using the LEICA Scan TCS-SP2 software (Leica Microsystems).
The ROS level was examined by detecting the fluorescence intensity using fluorescence microscope and microplate reader with excitation an emission wavelengths set at 500 and 529 nm, respectively.
After 24 hours, the solutions were transferred to 4-mL methylcrylate fluorescence cuvettes followed by fluorescence measurements using a Tuner Barnstead spectrofluorometer with excitation and emission wavelengths set to 490 nm and 520 nm, respectively.
The fluorescence of derivatized compounds (ATP, ADP, AMP, and ADO) was monitored with excitation and emission wavelengths set at 280 and 420 nm, respectively.
A fluorescence detector with excitation and emission wavelengths set at 385 nm and 500 nm respectively was used to quantify the reactants and products.
Extra-mitochondrial Ca2+ was measured in the presence of 1 µM Calcium Green-5N with excitation and emission wavelengths set at 506 and 530 nm, respectively.
Similar(27)
The injection volume was 20 μL and the detection wavelength set at 246 nm.
The detection wavelengths used were 210, 245, 280 and 365 nm with reference wavelength set at 700 nm.
A UV detector was used, with the wavelength set to monitor absorbance at 260 nm.
Cell optical density was read at 600 nm while GFP fluorescence was measured with the excitation and emission wavelength set at 488 nm and 508 nm, respectively.
Tyrosine was detected by fluorescence with the excitation wavelength set at 275 nm and the emission wavelength set at 303 nm.
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