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The interaction mechanisms between a pulsed laser and AuNPs are complex processes highly dependent on the pulse wavelength, duration, energy and intensity.
Plants can sense multiple aspects of the light signals including light quantity (fluence), quality (wavelength), duration (photoperiod) and direction [ 17], which are perceived through at least four different families of photoreceptors, including phytochromes (red/far-red light receptors), cryptochromes and phototropins (blue light receptors) and UV-B photoreceptor (UVR8).
Both acute and chronic UV exposure can lead to various ophthalmic abnormalities, associated with photochemical damage to cellular systems with the appearance of typical abnormalities; the type and extent of damage from UV radiant energy are associated with the wavelength, duration, intensity, and size of the exposure [ 23– 25].
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The ocular MPE of incident laser depends on the optical wavelength, pulse duration, exposure duration, and exposure aperture.
There is a lot of work and research on different wavelengths, durations and timing in their use.
The laser wavelength, pulse duration, and repetition rate were 532 nm, 5 ns, and 10 Hz, respectively.
Furthermore, germination recovery depends on the characteristics of UV treatment (wavelength, and duration of exposure) and the lapse time between UV treatment and subsequent exposure to optical radiation in the recovery range.
These states are populated differently depending on the light intensity, wavelength, and duration.
Regardless of whether the initial damage is caused by a photochemical effect, LED light damage is dependent on wavelength and duration.
The virus particle stability has been investigated under severe optical excitation upon varying several physical parameters (wavelength, pulse duration, energy/pulse, and repetition rate) with the precise aim to evaluate stability and tolerances to be used for optical spectroscopies.
Thus, further improvements are required, which could be achieved either by reducing the extent of matrix effects by using optimized instrumental parameters (e.g., laser radiation wavelength, pulse duration, robustness of ICP) or by developing alternative strategies for quantification.
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