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The occurrence of this 'second wave of stimulation' is quite evident from an elevated level of phospho-p38 MAPK at 24 hours in control cells.
PI3-K activity was inhibited at about 16 hours, and thus its effect on Akt phosphorylation occurs only with the second wave of stimulation.
The immunoblot in Fig. 6 also demonstrates that at 24 hours the levels of these phosphoproteins started to increase in the control cells, indicating the start of a second wave of stimulation.
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MySpace was no longer a site for sharing and discovering; it was a tomb filled with the mummified remains of people's profiles prior to the next wave of social stimulation that mercilessly continued to exist.
we allowed a second wave of cytokine stimulation.
In summary, a set of molecules is readily induced in T cells upon TCR signaling and provides a second wave of co-stimulation.
A set of co-stimulatory molecules is not constitutively expressed on T cells, but is rapidly induced upon activation and provides a second wave of co-stimulation [ 20, 43].
As I rocket around New Jersey Motorsports Park, this supercar physically compels me to switch off skepticism, pay heed to the racing line and happily marinate in waves of sensory stimulation: the Formula One shriek of its 552-horsepower V-10, the tightrope balance and technical precision that makes even a Corvette ZR-1 feel like a Tinkertoy.
ICWs were induced by focal mechanical stimulation of single astrocytes and the radius of calcium wave spread from the point of stimulation was measured before and after exposure to LDPBS (low-divalent PBS; containing nominally zero Ca2+ and 43 μM MgCl2, prepared by dissolving 1 mM MgCl2 and 1 mM EDTA in the Ca2+- and Mg+2-free DPBS), as previously described (Suadicani et al., 2006).
With the aid of the stepwise regression analysis method, the impacts of sound wave stimulation on the development of callus can be elucidated with a limited number of experiments.
Square wave current stimulation pulses of duration 100 µs were given at a frequency of 1 Hz.
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