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For solid phase extraction, C18 plus SepPak® cartridges were purchased from Waters (Waters® Associates, Milford, USA).
The concentrations were much lower as compared to those found in plants growing in wetlands receiving acid mine drainage waters, waters from smelters or highway runoff.
The analytical HPLC column for the optimized quality control (XBridge® Shield RP-18; 2.5 μm; 3.0 50 mm) and C18plus SepPak® cartridges for solid phase extraction (SPE) were purchased from Waters (Waters® Associates Milford, MA, USA).
HPLC chromatograms were recorded on a Waters model Alliance 2695 with Photodiode array detector 996 from Waters (Waters, Milford, USA) using a Symmetry C18 column (150×4.6 mm×5 µm, 100 Å, Waters) and a 717 Waters automatic injector, with a software Millenium version 4.0.
Alumina cartridges (Sep-Pak® Light Alum N) were purchased from Waters (Waters® Associates Milford, MA, USA).
The reagent is also commercially available from Waters (Waters corp., Milford, MA).
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