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After 2 h of hydrolysis, the reaction mixtures were heated for 10 min in boiling water to terminate the hydrolytic reaction.
The sections were placed in distilled water to terminate the DAB development reaction and mounted with 25% ethanol in PBS on to positively charged glass slides (ThermoFisher).
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The withdrawn aliquots of digestion mixture were diluted 18-fold with deionized water into sealed 2.0-mL HPLC vials, and then immersed for 10 min in a boiling water bath to terminate the enzyme reactions.
The withdrawn aliquots of digestion mixture were diluted 18-fold with deionized water in sealed 2.0-mL HPLC vials, and then immersed for 10 min in a boiling water bath to terminate the enzyme reactions.
After the experimentally desired time, the reactor was immersed into a cooled water bath to terminate the reaction.
The reaction mixtures were incubated at pH 6.0, °C °C for 12 h, followed by 10-min boiling water bath to terminate the reaction and centrifugation at 12,000× g for 5 min to remove unsolved residues.
The mixture was taken at appropriate times and treated in boiling water for 5 min to terminate the reaction.
The reaction conditions were: 63 °C-water bath for 60 min, then 80 °C-water bath for 2 min to terminate the reaction.
Although today's water buildup was much smaller, it was enough of a concern to terminate the mission early.
Each reaction was incubated at 62°C for 30 min and then 80°C for 5 min to terminate the reaction using a standard heat block or water bath.
tries to terminate the deal.
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