Exact(3)
Then, the gels were washed three times for 20 minutes in de-ionized water, sensitized for one minute with 0.02% sodium thiosulfate, washed thrice for 20 seconds in water and incubated for 20 minutes in silver staining solution (0.2% silver nitrate, 0.0074% formaldehyde).
Then, the gels were washed three times for 20 minutes in de-ionized water, sensitized for one minute in 0.02% sodium thiosulfate, washed three times for 20 seconds in water and incubated for 20 minutes in silver staining solution (0.2% silver nitrate, 0.0074% formaldehyde).
Then, the gels were washed thrice for 20 minutes in ultrapure water, sensitized for one minute in 0.02% sodium thiosulfate, washed three times for 20 seconds in water and incubated for 20 minutes in silver-staining solution (0.2% silver nitrate, 0.0074% formaldehyde).
Similar(57)
Then, the gels were washed once with distilled water and sensitized (150 ml ethanol, 34 g anhydrous sodium acetate, 1.57 g anhydrous sodium thiosulfate and metered to 500 ml with distilled water) RT for 30 min.
In a similar fashion, ethanol odor testing may have represented an additional stressor to water demonstrators already sensitized by the intubation procedure: thereby resulting in diminished odor responsivity relative to water observers.
After washing the fixed gels with distilled water, they were sensitized in 0.02% sodium thiosulfate.
Specifically, motion probing gradients are applied to non-directionally sensitize water molecules in order to determine water movement between diffusion-sensitizing gradient pulses [ 9].
Roxbury has already tried to buy more water from the county, meanwhile, but any expansion of the supply to Roxbury would come at a cost to other towns, or perhaps to the source aquifers themselves, and would require the approval of state regulators suddenly sensitized to water scarcity.
Such programmes should also ensure the availability and correct use of protocols for management of eclampsia, address other organizational barriers for the provision of quality health services such as shortage of staff, inadequate power and water supply and sensitize religious and village leaders on the advantages of facility-based child delivery to women, children, and communities.
Mice of wild type (BP-2, BALB/c, and C57BL/6) and mutant strains (lacking iNOS or CD95L) were given ovalbumin (OVA) or water (vehicle) orally and subsequently sensitized and challenged with OVA (OVA/OVA/OVA and H2O/OVA/OVA groups, resp .. Anti-OVA IgG and IgE, bone marrow eosinophil and neutrophil numbers, and eosinophil and neutrophil production ex vivo were evaluated.
Our DTI data was acquired with a b-value of 1250 s/mm2 and is thus sensitized to fast diffusing water protons (probably in the extra-cellular space).
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