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In the laboratory, water samples were prepared for nitrate determination using the cadmium reduction method and samples for TP analysis were prepared using the acid persulfate digestion method.
Drinking and reclaimed water samples were prepared by adding 10 μL of 500 ng/mL sucralose-d6 and the final volume was made up to 10.5 mL with the sample.
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Briefly, 10-fold dilutions of each water sample were prepared in phosphate-buffered saline (U.S. EPA 2006), and 10 mL of each dilution was filtered through a 0.45-μm, 47-mm mixed cellulose ester filter (Millipore, Billerica, MA).
Briefly, 10-fold dilutions of each water sample were prepared (10, 10−1, 10−2, and 10−3), and 10 mL of each dilution were filtered through 0.45-μm, 47-mm mixed cellulose ester filters (Millipore, Billerica, MA), which were placed onto appropriate agar plates.
Water and plasma samples were prepared using acetonitrile and an internal standard added prior to analysis.
The emulsions were prepared by consecutive addition of styrene, water, glycerol (some samples were prepared without glycerol), and SDS.
The samples were prepared, using water as solvent, at 100 μg/ml.
With distilled water as the base liquid, the samples were prepared with 0.5 wt.% MWCNTs and 0.25% GA and sonicated at various times.
Samples were prepared in water as previously described [68].
The samples were prepared in water to a final concentration of 0.8 1.0 mg mL−1, and pH was adjusted using dilute KOH or HClO4 solutions.
For sRNA sequencing, 7-day-old seedlings of Bre were root-inoculated with the bacterial strain (Erwinia carotovora subsp. carotovora), while seedlings inoculated with sterile water served as mock controls; RNA samples were prepared 2-weeks after inoculation [ 31].
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