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Water samples were kept at 4 °C till further processing.
All water samples were kept in a cool box containing ice packs and immediately transported to the laboratory for analysis.
The water samples were kept in dry place under normal atmospheric temperature and then analysed for pH, electrical conductivity and turbidity.
The water samples were kept in vials by adding Fe2O3 NPs and sealed with cap to avoid evaporation of water (Fig. 1).
Water samples were kept refrigerated, brought to the laboratory as soon as possible, and acidified to 0.5% V/V with ultrapure HNO3.
Every two weeks, water samples were collected in 1 L plastic bottles to determine suspended solids (inorganic and organic) nutrients, chlorophyll a. Water samples were kept on ice during transport to the laboratory.
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For the DBP precursor analysis, the pH value of water samples was kept at 7.0 ± 0.2 by first adding H2SO4 or NaOH (Merck, Germany) solution, and followed by adding adequate amount of phosphate buffer solution (0.8 M, Merck, Germany).
Post dose urine samples were collected after 5 hours and on day 3. Urine samples were kept in water-tight tubes, frozen at − 20°C, stored and transported to the Gas-Isotope-Ratio Mass Spectrometry Laboratory at the USDA/ARS Children's Nutrition Research Center at Baylor College of Medicine in Houston, Texas, USA for isotopic analyses.
The blood samples in lithium heparin and the urine samples were kept in iced water until centrifuged (1300 g, 10 min) at 4°C.
To evaluate the water quality, the samples were kept in polyethylene bottles and stored in insulated ice cooler that were delivered to the laboratory on the same day.
Concrete samples were kept in water for 28 days, then immersed in artificial sea water for 5 months.
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