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Chicken were immunized with rKLK6, the laying eggs were collected and IgYs were purified by a modification of the acidified water procedure [28].
Cortisol did not change in response to the warm water procedure.
Ratings of the participants in the warm water procedure were low (<10 on the scale of 1 to 100).
Daily total energy expenditure and body composition were measured in the field with the doubly labeled water procedure.
Then the experimenter led participants to a nearby room and collected additional saliva samples at 10, 20, 30, 45 and 60 min after the stress or control (cold or warm water) procedure was finished.
- Systolic and diastolic blood pressure (Table 1) increased significantly in response to the cold pressor test compared to the warm water procedure (mixed 2×3 ANOVA; systolic F 2, 116) = 35.96, p<.001, η2 = .38; diastolic F 2, 116) = 15.35, p<.001, η2 = .21).21
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The automatic watering procedure was monitored by a DL2e data logger (Delta T) with SM200 sensors connected to individual pots.
The trickiest bit is adding the lye to water, a procedure we perform in the open air so that the noxious fumes can blow away down the valley.
Thus, the water filling procedure is not applied here.
The lake water sampling procedure was demonstrated and volcanic features were explained.
In this paper, we describe and discuss the water sampling procedure.
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