Excess stain was rinsed by water, plates were air dried, dye was solubilized with 200 μL of 33% glacial acetic acid and OD of de-staining solution was measured using spectrophotometer [51].
At the start, everything we had came from ourselves, from our salaries and all sorts of contributions in kind: water, plates, tables, anything we needed, we found somewhere.
After two washes with water, plates were coated with 10 μg/mL of P8 peptide/well, and the plates were then incubated at 4°C overnight.
Earth is a really complicated place - it has water, plate tectonics, volcanoes, and more importantly, it has life.
Seeds were surface-sterilized for 2 min in 75% ethanol, followed by 5 min in 1% NaClO solution and washed five times in sterile distilled water, plated on growth medium (MS medium, 1.5% sucrose, 0.8% agar and pH 5.7).
Transformants were resuspended in water, plated on selective plates, and grown at 30°C for 2 4 days, and pinned to selective SC+Gal-Leu plates at 37°.
To measure the number of live Listeriaat bacteria, the infected cells were lysed in water, plated on agar, and counted for Listeriaat colonies the next day.
Medicago truncatula seeds (approximately 150 per batch) were scarified in concentrated sulfuric acid for 8 min, washed 3 times in sterile water, plated on 1% deionized water agar plates, and then stratified for 24 h or 48 h at 4°C and germinated by incubating at room temperature overnight [ 67].
To test the fraction of diploid cells from a colony on selection medium, colonies were suspended in water, plated on rich medium, and 100 single colonies were transferred to selective media.
The supernatant was tested for chemical parameters, and the pellet was resuspended in 100 μL distilled water, plated on RBCA medium and incubated at 30°C for 3 7 days, and then stored at 4°C prior to analysis.
With paper clip, face down the black paper on the surface of the plate & quickly take it out as, it will create the random mark of blue color on the paper which was on the surface of the water plate.
