"He gets a rash from water," Ma said.
"It is increasingly difficult to find clean water," Ma told me.
As we stood together looking into the water Mae's words were forever seared into my soul.
Each cell pellet was homogenized in lysis buffer (4 M Urea in 50 mM Ammonium Bicarbonate, 0.1% RapiGestTM; Waters, MA) and Roche protease inhibitor cocktail) and protein concentration was measured by the Qubit Protein Quantification Assay (Thermo Fisher Scientific, Rockford, IL).
The bioactive fractions were recorded on a HPLC MS/MS system which consisted of the HPLC Premier XE (Waters, MA, USA) connected through a split to the mass spectrometer (Waters, MA, USA) detector system operating at 45 °C.
Molecular weight of polymers was measured with the gel permeation chromatography (GPC) system (Waters 2690D-2410, Waters, MA, USA) as described previously [5].
UPLC/MS/MS analyses were carried out on an Waters Xevo TQ MS ACQUITY UPLC system (Waters, MA).
Analysis was carried out using reversed-phase HPLC (5 µm microbore, Phenomenex, Macclesfield, UK) and dual wavelength monitoring (Waters, MA, USA).
The supernatant (12,000 rpm for 10 minutes) was collected and injected (20 µl) into an HPLC system equipped with a fluorescence detector (Waters, MA).
ProteinLynx GlobalServer version 2.3 (Waters, MA, USA) was used to create peak list files.
