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After exposure, fish were transferred to clean water, grown to sexual maturity, and placed in mating pairs.
For chloronemal filament induction assays, protonemal tissue was subcultured at 7-day intervals on BCDAT medium, then protonemal tissues were diluted 1/20 in water, grown on BCD medium without ammonium tartrate and analyzed after 6 days of growth.
Similar(58)
Fresh water grows scarcer.
Water grows acid, eats stone, heats air.
In places, the water grew choked with lily pads.
Noncarbonated drinks, including Vitaminwater, Powerade and Dasani bottled water, grew 11percentt in the quarter.
As time went on, the lack of water grew increasingly dire.
The insects do not require much food or water, grow fast and reproduce quickly.
We set up our tent and watched the water grow dark.
As water grows scarce, special interests hurry to get it while they can.
The flow of water grows stronger as it heads toward Florida; it does not diminish.
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