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Three fluids were studied; water (for validation purposes), a power-law fluid (0.13 m/m% Carbopol 971 solution) and Bingham plastic fluids with Hedström number varying from 10 to 106.
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Due to differences in criterion methods there are limitations concerning comparability of studies since the doubly labeled water method used for validation of daily energy expenditure [ 11, 12, 30] does not allow for the activity specific validation attended in the current study design.
We took the opportunity of the dialysis centre being relocated and the need for water treatment validation to interrupt HDF during 6 months allowing a cross-over follow-up.
Additional measurements of reference materials air, water, ethanol and methanol are used for validation.
We also introduce and briefly present an iterative water routing method and use it for validation on a case with concrete terrain data.
Additional simulations are carried out using explicit solvent models with the CHARMM force field and explicit TIP3 water [24] implemented in NAMD [25] for validation of the effective Gaussian simulations.
Atomistic level molecular dynamics simulations are carried out using the CHARMM19 all-atom energy function with an effective Gaussian model for the water solvent [22], [23], as well as by using explicit solvent simulations with the CHARMM force field and TIP3 water [24] implemented in NAMD [25] for validation of the effective Gaussian simulations.
Doubly labeled water measurements of total energy expenditure were used for validation.
Precipitated DNA was resuspended in water and used for real-time quantitative (qPCR) (for validation of IP efficiency) or for microarray hybridisation.
Hence, the size of tanks or water bodies needs to be taken into account when using data for validation or parameterisation of a model.
For validation purposes, linearity was determined by adding standard solution of 25(OH)D2 and 25(OH)D3 to water and plasma.
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