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Wastewater was diluted to 25% with tap water for experiments with C. protothecoides and sea water for experiments with T. suecica.
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Dilution water for Experiment 1A was obtained from a well of approximately 40 m depth located on the WLI site.
The waste water used for experiments was arranged from Bhoramdev Sugar Industry Ltd.
Water used for experiments was distilled and passed through a Milli-Q purification system.
No attempt was made to mimic the elements or compounds present in natural groundwater in the water used for experiments, in order to ensure that results could be directly linked to experimental conditions, as possible side interactions could have occurred due to the presence of introduced solutes.
Stock solutions of carbon particles (20 mg mL−1) were dispersed in water (for the experiments shown in Figure 2 a) or buffer (for all other experiments, 50 m m Tris-HCl, pH 8) by sonication for at least 15 min. The required enzyme solutions were pre-mixed before the addition of an aliquot of the carbon particle suspension.
It is costly to provide formation water for the experiments while sea water is cheaper and more appropriate to conduct injection and imbibition tests.
After irradiation, the synthesized QDs were separated from the solution with a centrifugation at 10,000 rpm for 5 min. The samples were washed three times with deionized water and stored in deionized water for further experiments.
To characterize the long-term stability of Ag@SiO2 core-shell nanostructures in aqueous solution sensing applications, we put the Ag NP films coated with 0, 10, and 20 nm SiO2 layers into deionized water for comparison experiments.
Measurements were performed in ND96 low Ca2+ (5 mM Hepes, pH 7.5, 96 mM NaCl, 2 mM KCl, 1 m MgCl2) at 18 20°C using a Nikon Eclipse TE 300 microscope with a 20×0.75 NA multi-immersion (water for our experiments) lens.
T. orientalis extract was dissolved in water for animal experiments [ 8].
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