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Dilution water for Experiment 1A was obtained from a well of approximately 40 m depth located on the WLI site.
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A local laboratory was using tap water for experiments and had discovered compounds called bromates, which studies have associated with cancer.
Wastewater was diluted to 25% with tap water for experiments with C. protothecoides and sea water for experiments with T. suecica.
The reactor was loaded with deionized water and initial sewage sludge (2 wt % of deionized water) for every experiment (250 rpm and particles size 180 μm).
One hundred µL of vaterite microsphere stock solution was washed 2X and resuspended in 100 µL of Millipore water for each experiment.
Stock solutions of carbon particles (20 mg mL−1) were dispersed in water (for the experiments shown in Figure 2 a) or buffer (for all other experiments, 50 m m Tris-HCl, pH 8) by sonication for at least 15 min. The required enzyme solutions were pre-mixed before the addition of an aliquot of the carbon particle suspension.
It is costly to provide formation water for the experiments while sea water is cheaper and more appropriate to conduct injection and imbibition tests.
After irradiation, the synthesized QDs were separated from the solution with a centrifugation at 10,000 rpm for 5 min. The samples were washed three times with deionized water and stored in deionized water for further experiments.
To characterize the long-term stability of Ag@SiO2 core-shell nanostructures in aqueous solution sensing applications, we put the Ag NP films coated with 0, 10, and 20 nm SiO2 layers into deionized water for comparison experiments.
Measurements were performed in ND96 low Ca2+ (5 mM Hepes, pH 7.5, 96 mM NaCl, 2 mM KCl, 1 m MgCl2) at 18 20°C using a Nikon Eclipse TE 300 microscope with a 20×0.75 NA multi-immersion (water for our experiments) lens.
T. orientalis extract was dissolved in water for animal experiments [ 8].
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