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Every time Phelps dived into the water for a final here, the ripples extended into every corner of the Water Cube.
The family rejoined and returned to the water for a final drink together before departing as one large (and perhaps just a touch dysfunctional) family.
The different size nanofluids were centrifuged at 6,000 rpm for 30 min and re-dispersed with high-performance liquid chromatography (HPLC) water for a final concentration of 0.1 mg/ml.
Digested DNA was precipitated in SET and ethanol, and resuspended in water for a final concentration of 1 µg/ml.
The sample was quantitatively diluted to 5 mL using ultrapure water for a final concentration of 2% HNO3 and filtered through a 0.22 μm nylon syringe filter.
The amplification was performed in a solution containing 1× PCR buffer, 0.2 mM dNTP, 2.0 mM MgCl2, 0.75 U of recombinant Taq DNA polymerase (Promega), 10 ng of total DNA, 5 pmol of each primer and sterile Milli-Q water for a final volume of 25 µl.
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Forty microliters of the RT-PCR mixture and 10 µL of sample were dispensed into a 0.2-mL thin wall PCR tube, and 10 µL of Rnase-free water was added for a final volume of 50 µL.
Isolated genomic DNA (12.5 ng) was added to 7.5 μl mastermix consisting of Fast SYBR® Green I dye 2× (5 μl/reaction), forward and reverse primer (each 0.3 μl/reaction) and RNase free water (1.9 μl/reaction), for a final volume of 10 μl per reaction.
TaqMan reactions were carried out in 96 well plates using cDNA, TaqMan universal PCR mastermix, pre-optimized and pre-formulated TaqMan® gene expressions assays including specific primers and fluorescent probes for humans, and water to a final volume of 50 μL according to manufacturer's instructions.
KENNEDY SPACE CENTER, Fla .— The space shuttle Discovery is set to lift off Wednesday night on a two-week mission, carrying a replacement part for a water recycler and a final set of solar arrays for the International Space Station.
The shuttle is set to carry a replacement part for a water recycler and a final set of solar arrays to the station.
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CEO of Professional Science Editing for Scientists @ prosciediting.com