Exact(17)
Without liquid water, cells fail, and so do those functions that define us.
When the feeding is good they accumulate stores of fat in their humps, upon which they are able to draw when conditions are adverse not only for sustenance but also for the manufacture of water by the oxidation of the fat; but they do not store water in the miscalled water cells.
After washing three times with deionized water, cells were stained with Oil Red O in isopropyl alcohol at a concentration of 4 mg/ml.
After a wash with water, cells were stained with 60% filtered Oil-Red-O stock solution (0.5 g of Oil-Red-O (Sigma) in 100 ml of isopropanol) for 1 hour at room temperature.
Lipid accumulation in adipocytes was visualized by staining with Oil Red O. Briefly, differentiated adipocytes were washed with phosphate buffered saline (PBS) and fixed with 10% formaldehyde in PBS for 4 min. After two washes with water, cells were stained for 1 2 hrs with Oil Red O working solution (0.3% (wt/vol) in 60% isopropanol).
After three more washings with deionized water, cells were scored for adipocyte differentiation and photographed.
Similar(43)
Any two-state solution with a chance of working would have to include federal arrangements not only about security but also about water, cell-phone coverage, sewage, and countless other details of a common infrastructure.
To evaluate the stability of rNGO-PEG/ICG, we monitored the dispersions of rNGO-PEG/ICG in water, cell medium (DMEM), fetal bovine serum (FBS), and PBS solutions.
Irradiation was filtered by a circulating water cell (thickness 5.0 cm) to remove IR beams thus preventing any heating of the suspension.
"If you think about things we take for granted that are infrastructure — electricity, water, cell phone plans, phone plans, you name it — these are things you pay for, and you expect that when you turn on the faucet the water comes out, and that it is what you expect.
For weeks ― and in some cases, for months ― many Puerto Rican households were without access to medical services, water, cell phone coverage and electricity.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com