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Figure 2 Chromatogram and mass spectrum of stanozolol spiked to tap water at a final concentration of 0.5 pg/mL.
Figure 1 Chromatogram and mass spectrum of stanozolol spiked to Danube river water at a final concentration of 0.5 pg/mL.
SU5402 (Calbiochem) was dissolved in DMSO and added to the fish water at a final concentration of 17 µM (0.01% DMSO) at 5 hpa.
Stock solutions of BSF (purified from FBS; obtained from Sigma) or HSA (purified from HS; Sigma) were prepared in water at a final concentration of 20 mg/ml.
They were dissolved in water at a final concentration of 1% (w/v) and sonicated for 20 min (aquasonic, model 50 T, VWR, NJ).
THZ (Sigma), dissolved in water, at a final concentration of 1xMIC (10 µg/ml) or 4xMIC (40 µg/ml) was added to Mtb (CDC1551) cultures grown in Middlebrook 7H9-albumin-dextrose-catalase-Tween 80-glycerol broth at an OD546 of 0.5 - 0.6 and the cultures were aerated by shaking at 37°C for additional 1 6 h.
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The microcosm water system at a final concentration of approximately 10 CFU/mL was used to starve S. typhi cells.
To prepare the aqueous extract, fresh seeds weighing 50 g were cleaned in a water stream, extracted with 500 mL of Milli-Q water, and concentrated at a final volume of 14%.
A 100 µM stock solution of 7-hydroxy-9H-1,3-dichloro-9,9-dimethylacridin-2-one (DDAO; Invitrogen, Karlsruhe, Germany), in demineralised water, was used at a final concentration of 4 µM.
Cycloheximide was dissolved in water and used at a final concentration of 2.5 µg/mL (Calbiochem), rapamycin was dissolved in DMSO and used at a final concentration of 100 nM (LC laboratories), TNFα was dissolved in PBS containing 0.1% BSA, and used at a final concentration of 10 ng/mL (R&D systems).
(Malvaceae) was dissolved in 10 % dimethylsulfoxide (DMSO) in water [ 20] at a final concentration of 400 μg/ml.
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