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After being cooled to room temperature, the mixture was washed three times using acetone and water alternatively.
The samples went through centrifugation of 3000 rpm for 10 min and re-dispersed in deionized water alternatively to remove biomass in the reaction solution.
Colchicine (C9754; Sigma-Aldrich) was resuspended at 10 mg/ml in distilled water (alternatively, media can be used) and used at 4 mg/ml.
This is attached via a plastic tube to an irrigation bag holding up to 2 l of water; alternatively a low-volume system consisting of a hand pump and a cone may be employed.
Clean them in a basin of cool water alternatively.
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A reverse osmosis unit can be used to filter the water or, alternatively, bottled distilled water can be purchased.
We hypothesized that homogeneity among the complaints might provide justification for a cross-sectional study of water customers; alternatively if we could not identify homogeneity, this might indicate the lack of a common cause, reducing the pressure for further investigation.
It is rapidly oxidized to Cr (VI) in acidic soils in the presence of Mn, which becomes insoluble in water and alternatively has adverse effects on plant growth and development [ 8].
Pneumonia results from haematogenous spread of B. pseudomallei to the lung following inoculation through exposure to muddy soils or surface water or alternatively by inhalation, as the two main modes of infection [ 1, 8].
Read the fabric instructions on clothing items before using this method; some fabrics cannot tolerate hot water or, alternatively, the borax might react poorly with the fabric.
You could alternatively run the washcloth through hot water instead.
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CEO of Professional Science Editing for Scientists @ prosciediting.com