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These segments were placed on water agar plates and were incubated at 28 °C.
High 500 mL beakers were filled with 100 mL water agar (1.0% agar-agar).
In each case three stalks of the treated leaves were drilled into the solid water agar.
Fungal mycelia growing from diseased tissue were transferred to 2% water agar.
The mushroom cap might be laid directly onto filter paper, aluminium foil or (water) agar.
They were pre-geminated for 48 h at 25 °C in Petri plates containing 1% (v/v) water agar.
The upper surface of the water agar was covered with sterile filter paper to prevent the larvae from getting stuck.
Conidia of eight- to nine-day-old colonies were dusted on water agar surface in Petri dishes and exposed to UV treatments (without lid).
The conidial suspension was then spread on water agar and incubated at 28°C for 4 h to test their viability.
In previous work, these strains have colonized more than 80% of the M. javanica eggs on water agar (Ebadi et al. 2009 and Moosavi et al. 2010).
The suspension was passed through a 70-μm sieve to remove mycelial fragments, and 100 μl of the resulting filtrate was spread on a 2% water agar plate.
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