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I was about 100 feet or so away and was standing in about a foot of water, adjusting my lenses for that perfect shot, when I spotted something out of the corner of my eye.
BSA solutions were prepared by dissolving 10 g of BSA in 100 mL of sterile deionized water, adjusting the pH to 7.4, and filtering the solution through a 0.22-μm pore membrane.
Running buffers were prepared by dissolving the appropriate amount of sodium phosphate in Milli-Q water, adjusting the pH with 1 M NaOH before completing the volume to get the desired buffer concentration (25 mM).
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Those who depend on the snowpack for water adjust their plans accordingly.
He slipped into the warm water, adjusted his mask and scuba gear, steadied his breathing and slowly began his descent several meters below, where the river water gradually turns from pale to thick, acidic yellow.
But the primary impression was of a man and a woman undergoing ritualistic purification, their gradual progress towards the camera, stripping away of clothes, washing and immersion in water adjusted to coincide in time with the live performance.
The filtrate was then poured into water, adjusted the pH to 9 usingammonia, and extracted with CHCl3.
Albumins were extracted with water (adjusted to pH 8.0) containing 10 mM CaCl2 and 10 mM MgCl2 (340 µl/10 mg) for ½ h.
Spore suspensions were prepared in sterile water, adjusted to OD530 = 0.1.
Briefly, stools were diluted 10 times in a dilution buffer (Na acetate 15g, acetic acid 3,60 ml, in 1000 ml water adjusted at pH5).
Perhaps the simplest in vitro method is to mix the food with water, adjust the pH to 2 (approximate gastric pH), incubate with agitation for a period of time, centrifuge, and measure the concentration of the iron in the supernatant.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com