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These observations demonstrate a strong link between overflow metabolism of acetate and carbon wasting into other products.
Carbon wasting into by-products increased from 3 to 11% in the carbon balance within the studied μ range.
This leads to potassium and magnesium wasting into the cortical collecting duct as the increase in delivered sodium is reabsorbed through the epithelial sodium channel.
An important central carbon metabolism branch point flux, pyruvate dehydrogenase reaction, reached its maximum throughput at μ = 0.42 h-1 with concomitant slight increase in glycolysis fluxes resulting in accelerated carbon wasting into by-products.
What is more, overall carbon wasting into the carbon wasting products in the carbon balance was similar (ca 5.5%) under very different maximal glucose-acetate co-utilisation capability values.
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