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Temperature had the highest effect, followed by lime to iron waste ratio.
The feed mixed waste ratio (FW/SWS) of 10 1 by weight was selected from the previous laboratory-scale experiment.
Molecular analyses demonstrate that both temperature and manure-to-food waste ratio greatly influenced the bacterial communities, while archaeal communities were mainly influenced by temperature.
The optimized conditions for maximum yield of carotenoids were 60% hexane in solvent mixture, solvent mixture to waste ratio of 5 1 in each extraction and three extractions.
Temperature, solid to liquid ratio and stirring speed were varied in addition to lime to iron waste ratio in optimization tests that followed.
The optimised conditions for maximum carotenoid yield (37.5 mg kg−1 dry waste) were 45% hexane in solvent mixture, solvent mixture to waste ratio of 9.1 1 (v/w) and particle size 0.56 mm.
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Two temperatures and three manure-to-food waste ratios were investigated by a completely randomized design.
The composting reactors were operated at 18 different operational conditions using a 3-factor factorial design with three temperatures (T, 38 °C, 55 °C and 70 °C), four soil to green waste ratios (S GW, 0.6 1, 0.7:1, 0.8 1 and 0.9 1 on a dry weight basis) and three moisture contents (MC, 40%, 60%and80%0%and80%
The composting reactors were operated at 18 different operational conditions using a logistic three-factor factorial design with three temperatures (T = 38, 55 and 70 °C), four soil to green waste ratios (S GW; 0.6 1, 0.7:1, 0.8 1 and 0.9 1 on a dry weight basis) and three moisture contents (MC; 40%, 60%and80%0%).
A regression equation for predicting the carotenoid yield as a function of three processing variable (hexane % in solvent mixture, solvent-to-waste ratio and number of extractions) was derived by statistical analysis, and a model with predictive ability of 0.98 was obtained.
An operation with a suitable sludge wasting ratio seems to be necessary to prevent accumulation of inorganic and inert particles for long-term operation.
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