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Indeed, we have previously demonstrated that PEG 35 addition to washout solution protected the liver against reperfusion injuries [ 27].
The dilute sulfuric acid-pretreated CS was thoroughly washed with deionized water until no inhibitor substances from the washout solution were found on performing high performance liquid chromatography (HPLC).
Rinse in water to remove the last of the washout solution.
Place the polymer image in the washout solution and gently scrub with fine bristle brush or soft toothbrush until all of the excess liquid polymer is dissolved from the stamp surface.
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The analysis showed that the system has two qualitatively different solutions (washout and culture survival), and that there are regions in the operating parameter space where multistability occurs.
The reduction in open probability could be reversed by washout of the gluconate solution (Fig. 3).
In all cases, acid-mediated effects were reversible after a 30-s washout with pH 7.4 solution.
Dye leakage from impaled cells is unlikely to cause significant labelling due to washout via the perfusion solution and to negligible labelling by extracellular membrane-impermeant dye; labelling of brain slices caused by micropipette insertion or by damaging an impaled cell is very low.
This rapid washout was not limited by the solution flow within the recording chamber, because only tens of seconds were required for complete exchange of the solutions.
Following baseline measurements, cells were exposed to the induction cocktail for 15 min, followed by washout to normal bath solution.
In multiple experiments, we observed full recovery of contractile force upon restoration of 2.5 mM [Ca2+]o by washout of EGTA-containing solution (n=18/20) (Fig. 2B).
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