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The accumulation of -[125I]IV-OH and -[125IVp IV was remarkably lessened in the presence of a sigma ligand, haloperidol at 10 μM in culture medium (Figure 3A). Figure 3B shows the percentages of radioactivity of -[125I]IV-OH and -[125IVp in in DU-145 cells to the time point of medium replacement in the washout experiment.
Finally, removal of released amino acids in a washout experiment abrogated inosine-mediated germination of B. cereus spores.
A washout experiment confirmed that 6 hour exposure of VER-150548 was sufficient to induce H2AX phosphorylation and that this was maintained for at least 18 hours after the removal of VER-150548 (Figure 7C).
We also calculated the apoptotic index in the washout experiment.
Given that Grx1-roGFP2 reacts with glutathione with a Kmax 0.5 in the 10−5 M range the effacement of its kinetic advantage over roGFP2 in the DTT washout experiment, indicated a profound and selective depletion of lumenal glutathione by the ER-targeted expression of active ChaC1, with modest effects on other cellular pools of glutathione.
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The composition, morphology and mechanical stability of the coatings were analyzed and the Sr release profiles were investigated in vitro by washout experiments.
Actually, in the washout experiments in the cell uptake study, more -125I]IV-OH was released from the DU-145 cells compared to that of -125I]p IV after changing to fresh media (Figure 4B).
In washout experiments using CLL tumor cells, the 48-hr cytotoxic effect of AR-42 was eliminated when the drug was removed after 4 hr (n = 5; p = 0.266 relative to the untreated control).
This effect was reversible because the levels of cholesterol after washout experiments were restored with both inhibitors but in higher levels with Dynasore than with M βCD.
Vrije et al. [ 21] found different values of μmax obtained in washout experiments with C. saccharolyticus, depending on the growth history of the culture.
In the case of the washout experiments with CA200645, the dissociation of the ligand was sufficiently slow (Fig. 5) to allow the contribution of ligand association to τD2 to be largely discounted.
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