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In the present work, a new particle classifier equipped with a novel symmetric quintuplet nozzle for washing stream has been introduced and evaluated experimentally.
This specific red-oil fouling can be successfully mitigated by regularly washing the caustic scrubber with a hydrocarbon washing stream which acts as a solvent.
Furthermore, the washing stream could be reused at an earlier stage of the process.
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However, such washing streams can be further rerouted to the quench water system, increasing the risk of emulsion formation due to its amphiphilic components, i.e., red-oils, compromising the safe operation of the whole plant.
The DMSO diffuses from a cell suspension stream into a neighboring wash stream flowing in parallel.
However, a greater cell density was achieved in most of the wash stream-containing fermentations relative to the control (YEP with no wash stream; Figure 3A).
The wash stream contains less than 2.5 g/L of monomeric glucose and xylose.
The time courses of KO11 and 424A LNH-ST) fermentations in both wash streams also showed similar trends as the microplate fermentations regarding strain robustness of 424A LNH-ST) and better xylose fermentationsof KO11 in AFEX-CS wash stream.
Fermentations by these three strains exhibited similar patterns; in that moderate levels of AFEX-CS wash stream improved cell growth but the degree of improvement decreased as the strength of the wash stream increased.
Potentially, the DA-based process can utilize the xylose-rich residue derived from pretreatment wash stream during fermentation.
While better xylose fermentation was achieved in KO11 than 424A LNH-ST) in AFEX-CS wash stream, the opposite trend was observed in dilute acid-CS wash stream due to the inhibitory nature of the wash stream and the strain robustness toward the inhibitors.
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