Washing, sonication and immunoprecipitation were performed as described previously.
For each chromatin sample, 100 male flies were homogenized in PBS, and cross-linked in 1.8% formaldehyde at RT for 10 min. Cross-linking was stopped by addition of glycine (final concentration 0.25M) for 5 min. Subsequent washing, sonication and chromatin immunoprecipitation followed the protocol described in [ 23].
The actual effect of filtration washing and sonication dispersing process could improve the conductivity of AgNW films [15]; however, it is too time-consuming to produce nanoscale ink by filtration in practical applications.
After washing and sonication, bacteria (5,000 cells/determination) were analyzed in a flow cytometer (Becton Dickinson, FACSCalibur flow cytometer, Heidelberg, Germany) using an excitation wave length of 488 nm at the FACSCalibur standard configuration with a 530 nm bandpass filter.
In a typical process, the pieces of the selected silicon wafers were washed by sonication in acetone and deionized water.
After the reaction, the electrode was washed by sonication in ethanol for 5 min and rinsed with water.
Previous work by Zhang et al.[18] demonstrated a similar MTT concentration-dependent viability profile with neural phaeochromocytoma-derived PC12 cells exposed to graphene synthesized via CVD (purified using a diluted hydrochloric acid wash with sonication).
After that, slides were washed by sonication for 10 min in sterilized DI water.
Chromatin and antibody bead complexes were formed during at least 2 h followed by 5 min washes with sonication buffer (50 mM Hepes, 140 mM NaCl, 1 mM EDTA, 1 % Triton, 0.1 % sodium deoxycholate, 0.1 % SDS), WashA (as sonication buffer, but with 500 mM NaCl), WashB (20 mM Tris pH 8, 1 mM EDTA, 250 mM LiCl, 0.5 % NP-40, 0.5 % sodium deoxycholate) and TE.
It was observed that the PEMs were selectively deposited on the EAA layers and linked through covalent bonds, but those on LLDPE layers were washed off during sonication in water and methanol.
Immediately after treatment the biofilm cells were detached via sonication, washed with 0.85% NaCl buffer, and plated on LB-agar plates to quantify the number of viable cells by counting CFUs.
