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Percentage oospore germination was determined by placing a 0.1 mL sub-aliquot of each bark washing sample in the well of a cavity microscope slide.
Separate methods have been produced and tested that automate the sample loading, column washing, sample elution and peak collection steps for ion exchange, metal affinity, hydrophobic interaction, and gel filtration chromatography.
However, M. genitalium was found in only 1 throat washing sample and was not detected in throat washing samples obtained from 403 FSWs in our previous study (11 ).
In summary, we found that obtaining additional second bronchial washing sample increased the diagnostic yield by up to 13% under tolerable adverse events.
M. genitalium was also detected in a throat washing sample from 1 FSW (0.7%, 95% CI 0%2.00%), whose vaginal swab sample was negative for M. genitalium.
Of 101 patients (171 clinical samples) showing negative mycobacterial culture and AFB smear, there were five (9 sputa samples and one bronchial-alveolar washing sample) that showed inconsistent results with hsp65 Nested PCR-PRA.
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After washing, samples were analyzed by flow cytometry.
After PBS washing, samples were embedded in paraplast.
After PBS washing, samples were mounted in Vectashield with DAPI (Vector).
After each washing, samples were centrifuged at 50,000 g for 30 min at 4°C.
After washing, samples from the immunoprecipitation were run on an SDS-PAGE.
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