They noted that less than 2 percent of hospitals had installed comprehensive electronic medical records systems, doctors and nurses were routinely working in excess of limits on duty hours and few were paying attention to even simple hand-washing recommendations.
The glass milk was washed in 1 ml of washing buffer following manufacturer recommendations.
Cells were then stained with intracellular anti-Foxp3-Pacific Blue (clone PCH101) (eBioscience) as per the manufacturer's recommendations, followed by washing and fixation in 1% paraformaldehyde.
Fragment labeling and hybridization were done using the AlkPhos Direct Kit (Amersham Biosciences), according to the manufacturer's recommendations (hybridization and washing temperatures varied between 50°C and 61°C according to probe AT/GC content).
"That can cover anything from washing instructions to helping provide wardrobing recommendations," he noted.
RNA was extracted by phase separation, precipitation, washing and redissolving according to the recommendations of the manufacturer (TRIzol, Invitrogen Life Technologies).
Slowinski writhed in pain as his eyes were washed, on the recommendation of Burmese villagers, with the juice of tamarind leaves.
Labeled cRNA was purified, fragmented and hybridized to Xenopus tropicalis Genome Arrays (Affymetrix) followed by washing and staining steps according to the manufacturer's recommendation.
The MOE430A and B chips were hybridized to the fragmented cRNA, stained and washed according to the recommendations of the Gene Chip® Expression Analysis technical manual (Affymetrix, Santa Clara, CA) in the Purdue University Genomics Core facility.
1.5 μg of cRNA (> 9 pmol Cy3 per μg cRNA) was fragmented, hybridized to Agilent Whole Human Genome Oligo Microarrays (G4112F) using the Gene Expression Hybridization Kit, and washed following Agilent's recommendations.
