"Every prisoner has to begin detention sober and washed," reads the accompanying text.
Examples of 'doing' are travelling, going to work, washing and reading.
After washing, the reading buffer was added and the ECL signal was measured with the SECTOR Imager 6000.
After washing, the reading buffer was added and the ECL signal was measured with the SECTOR Imager 6000 Meso Scale Diagnostics, Rockville, MD, USA.
The Aβ 3-Plex plates were incubated for 2 hrs at room temperature with orbital shaking followed by washing and reading according to the manufacturer's instructions (SECTOR® Imager 2400, MSD).
Because of this higher level of automation (i.e., adding reagents, washing and reading plates), the total analysis time of Simple Plex assays was found to be much shorter than either Bio-Plex® or Multi-Array assays (1.5 h versus 4 6 h) (Table 1).
Samples were transferred to the blocked plates with 6E10 detection antibody and incubated for 2 hrs at room temperature with orbital shaking followed by washing and reading according to the manufacturer's instructions (SECTOR® Imager 2400 Meso Scale Discovery, Gaithersburg MD, USA).
Cells were washed before reading of their radioactivity; a direct measure of proliferation was performed in a beta counter (TopCount NXT, Perkin Elmer, USA).
An "extract" from the guide to literal brain-washing reads: "Always wear woollen gloves (or mittens) After the child's brain has been removed with the two brain spoons, rinse it in a solution of vinegar, ammonia and curry powder, then rest the brain on a soft cloth or tea towel for a few minutes, or for as long as is convenient".
If using face pads or washes, read and follow the directions on the packages.
