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In the other experiment, HeLa cells were incubated with Stx (10 µg/ml) for 10 min at 37°C, and after washing further incubated with anti-Stx antibody for 60 min on ice.
The transfected cells were incubated with 16-biotin or 16-dexran-biotin and, after washing, further incubated with phycoerythrin-conjugated streptavidin (saPE).
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For removing the colour of raw adsorbents, the grinded potato peel is dipped in 6 % HCHO overnight and washed further with distil water until the decanted water became colourless and pH reached at neutral.
After 3h at 37°C, cultures were washed, further incubated in fresh medium for 3 days, and fixed in methanol.
The lack of inhibitory activity when erythrocytes were pretreated with drug (and then washed) further confirmed specific inhibition of merozoite invasion (Fig. 5e; P <0.01).
Cells were permeabilized with 0.2% (v/v) Triton X-100 for 20 min and then washed further three times (5 min each) with PBS.
Each wave washing them further from political ideology.
Though Texans need only worry about gators washing up further inland, the opposite has been known to happen, too.
The first wash diminished the protein content of the resuspended LDL sample by 20%%, whereas the second wash further decreased protein content by 3%%.
The antimicrobial wash further led to a significant decline in in-vitro bone formation (bone nodule formed in-vitro) compared to saline solution [ 5].
Cells were washed a further two times with PBS before a brief incubation with 5 μg/ml Hoechst 33342 (Sigma) and further washes in PBS.
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