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Heat 100 mL HAuCl4 solution (0.01 %) to boiling and add 2.7 mL trisodium citrate (1 %) for 10 min, stirring frequently, followed with washing, filtering, and drying, and after that we get the AuNPs. 1 mL AuNPs solution and 6 μL TCEP were added into 50 μL MUC1 ampters (10 μM), and then stirred for 2 h.
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The pigment thus obtained is precipitated with dilute hydrochloric acid and is then washed, filtered, and dried.
Finally, the samples were washed, filtered, and then dried at 50°C in air atmosphere before characterization.
Regenerated biomass was washed, filtered and finally dried at 60 °C to calculate the loss in weight.
After a further 30 min at 4°C, the cells were washed, filtered and resuspended at ∼2×107 cells/ml in PBS 2% FBS before sorting.
After pretreatment, the cellulose rich water insoluble components were washed, filtered and refrigerated for long-term storage.
After pretreatment, all substrates (solid fractions) were thoroughly washed, filtered, and kept in refrigerated storage until they were used for analysis and hydrolysis.
All washed filter paper samples were covered with a layer of carbon (3 nm) and, subsequently, with a layer of PtPd (3 nm, 80%to20%0%).
The solvents were evaporated and the resultant solid in the aqueous layer was washed, filtered, washed with H2O, Et2O, EtOAc and dried in vacuo to afford compound 9 e as a white powder (90 mg, 74 %), mp: 201 203 °C.
After washing, filter was dried by spinning for an additional 1 min at 10,000 g.
After washing, filters were exposed for up to 3 d at −70°C with 2 intensifying screens.
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