Sentence examples for washing detection from inspiring English sources
The washing, detection and microscopic observation steps after hybridization were performed as described previously with slight modification (Sakurai et al. 2001; Ohashi et al. 2015).
After washing, detection was performed by fast red staining and counterstaining with hematoxylin.
After washing, detection was achieved with amino ethyl carbazole (AEC) for 5 10 min.
GST eEF2K[1 402] His6 was then added, and after extensive washing, detection was with Ni-NTA HRP coNi-NTA HRP
After extensive washing, detection of bound antibodies was carried out using horseradish peroxidase-labeled secondary antibody and enhanced chemiluminescence (Amersham).
After washing, detection of the protein bands was performed using the Femto kit (SuperSignal West Femto kit, Thermo Scientific, Erembodegem-Aalst, Belgium).
After four washes, detection was performed using TMB Liquid substrate system (Sigma Aldrich), 100 μL per well; the reaction was stopped with 1 M HCl, 100 μL per well.
The secondary antibody used was once again the goat anti-mouse IgG (1∶2000) for 1 hour at room temperature, followed by washes, detection with the ECL kit, and autoradiography.
After the final wash, detection was performed by enhanced chemoluminescence (Amersham, Freiburg, Germany).
The plate was then washed, detection antibody added, and incubated again at 37°C for 1 h.
After a second wash detection antibodies were added for 2 hours at room temperature.
