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Pigment-protein complexes were collected by washing column with eluting buffer: 0.5 M imidazole, 20 mM phosphate buffer pH 7.5, 0.2 M NaCl, 0.06% β-DM; LHCII trimers were separated from monomers by sucrose gradient ultracentrifugation [ 19].
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2. Prepare seal wash, needle wash, and column wash solvents as previously described (step 2, COX assay).
Design and scale-up rules for the purification of pastilles in a gravity wash column are formulated.
The applicability of this type of wash column, i.e. for large-scale ultrapurification of organic crystals, depends amongst others on the compressive stresses that are exerted on these crystals upon processing in a hydraulic wash column.
A new melt crystallisation process based on pastillation and purification in a gravity wash column has been tested on lab scale.
From calculations based on experimental data, rules were derived on how to minimize the compressive stresses during the operation of a hydraulic wash column.
In this paper an analytical model is presented which can be used in technical feasibility and scale-up studies to predict the compressive stress profiles that occur in a hydraulic wash column during operation.
Wash column x3 with 1 ml 80% ethanol.
Using a multichannel pipette, samples were washed column by column, starting with column 1.
Hydraulic packed bed wash columns are continuous solid liquid separators, which combine a virtually complete crystal mother liquor separation with a highly efficient washing action at high throughput.
In this paper an experimental study and a mathematical model of the purification process in packed-bed wash columns are presented.
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