Sentence examples for washing assay from inspiring English sources

After washing, assay standards (KLK6 recombinant protein produced in-house (Diamandis et al, 2000)) or ovarian cytosolic extracts were added to the wells in duplicates (100  μl well−1) after being diluted two-fold in assay buffer (50 m M Tris-HCl buffer, 6% bovine serum albumin, 0.01% goat globulin, 0.005% mouse globulin, 0.1% bovine globulin, 0.5  M KCl, 0.05% sodium azide, pH 7.8).

The adhesion strength of the keratinocytes on the surfaces was investigated using the washing assay.

To investigate the effect of bacteria on adhesion strength of keratinocytes, the washing assay was also applied to cells previously exposed to the bacteria.

After washing the assay was developed with 1 mg/ml p-nitrophenyl phosphate in diethanolamine substrate for 2 hours, fixed with 3 M NaOH and read at OD 405 nm and 690 nm.

MPs were isolated using a magnet, and unbound material was removed by washing with assay diluent using a HydroFlex plate washer (Tecan Group AG, Männedorf, Switzerland).

The alternative is binding of nonradioactive photoaffinity ligand to membrane receptors, followed by UV irradiation, washing, and assay of residual available receptors.

In contrast, washing the assay plates with Tween 20 or Tween 80 decreased the apparent titers in serum from the rabbit immunized with mPEG-uricase only c. 8-fold or c. 5-fold, respectively (black arrows).

After removing the non-adherent cells and washing by assay buffer, cells were fixed and stained for 5 minutes, after washing 3 5 times with deionized water, the cell-bonded stain was solubilized and quantified with an ELASA plate reader (Benchmark Plus microplate spectrophotometer, Bio-RAD, USA), at 560 nm.

Adhesive and invasive growth on agar were assessed by the plate-washing assay [ 48].

These dyes can exhibit scarce fluorescence in polar solvents but become highly fluorescent in weakly polar solvents, so they could be the ideal candidates for a no-wash assay.

The configuration of the fusion probes and the labeling scheme were optimized for detecting native receptor on the A431 cell surface in a no-wash assay format suitable for flow cytometry and fluorescence imaging.