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After washing again with PBS, Dnase (5 μg/ml) was added and incubated for 1 h at 37 °C.
After washing again with ethanol, the precipitate was taken into a heater which was maintained at about 60°C in air, and then finally, a white fine powder was obtained.
I kept washing and washing again and again … and finally, after about ten minutes of washing, I could get all the blood out of my hair.
Briefly, paraffin sections were deparaffinized, washed in PBS 3 time, followed by incubation with 3% H2O2 for 15 minutes and PBS washing again.
After washing again in PBS, sections were treated with peroxidase substrate to visualize staining, washed in distilled water and counterstained with Nuclear Fast Red.
EBs were isolated on different days over a 20 day period and fixed by washing with 1X PBS at room temperature (RT), incubating in 4% paraformaldehyde (PFA) for 30 min at RT, washing again with 1X PBS and then adding 80% ethanol prior to embedding.
Similar(17)
It would have to be washed again.
"I was afraid I would never wash again".
Then, washed again with distilled water.
The cells were washed with PBS, fixed, and washed again.
After that, the solutions were aspirated and washed again.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com