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After reaction accomplishment, the black powder was isolated by centrifugation, washed with alcohol and acetone several times, and dried at 50 °C for 24 h.
The treated sample was then washed with alcohol to remove the excess amine followed by washing with distilled water to remove the alcohol present.
The product was recovered by filtration, washed with alcohol and then was dried in oven under vacuum at 40°C for 48 h to give a yellowish amorphous product (Scheme 1).
Afterward, the swollen CPS beads were washed with alcohol and ultrapure water three times and then separated from the supernatant by filtration; (ii) 4.0 g of sodium hydroxide was dissolved in 100 ml of ultrawater, and 10 g of PEI was added to this solution.
Afterward, the swollen CPS beads were washed with alcohol and ultrapure water three times and then separated from the supernatant by filtration; 4.0 g of sodium hydroxide was dissolved in 100 ml of ultrawater, and 10 g of PEI was added to this solution.
As much blood as possible would then have been squeezed out of the corpse, and the whole body washed with alcohol.
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After washing with alcohol, the RNA was eluted in RNAse free water.
5 Athletes should be advised to seek shade and to hydrate regularly, to drink only bottled water, to eat safe food and to employ regular hand washing with alcohol gel.
All the patients were orotracheally intubated; VAP prevention measures included hand washing with alcohol based antiseptic, a 30° semi-recumbent patient position, MV with positive end-expiratory pressure >5 mmHg, and oral care based on local application of povidone-iodine solution six times daily [ 15].
Briefly, tissue was homogenized in Trizol; nucleic acid was separated with chloroform at high speed centrifugation; and RNA was then precipitated with isopropyl alcohol and washed with 70% alcohol.
Don't reenter poison ivy after you've washed with rubbing alcohol, however, as the alcohol dries your skin and removes its protective oils.
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