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"It had an empty stomach that looked like it had been washed with acid," she said, adding that otherwise it seemed well fed and healthy.
Glassware used for analysis was washed with acid solution followed by distilled water.
After crushing it was washed with acid to remove surface oxides using a protocol described in earlier work [4].
The sample obtained was washed with acid (0.2 N HCl) repeatedly until it reached pH 2 and then with aquadest until approximately pH 5. TiNT powder was then obtained after drying at 80 °C for 6 h and calcination at 500 °C for 1 h.
After 1 hr infected cells were washed with acid glycine for 3 min at RT (0.14 M NaCl, 5 mM KCl, 1 mM MgCl2-6H2O, 0.7 mM CaCl-2 H2O, 0.1 M glycine, [pH 3]) [93] to remove adherent virus and any viral particles in the media.
Cuvettes and stir bars were washed with acid and blocked with BSA as described.
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The precipitate was washed with acid-salt wash reagent and dissolved in alkali reagent, and then the absorbance at 555 nm was determined in a microplate reader (Bio-Rad Laboratories).
After 3 times washing with acid alcohol buffer, embryos were incubated for 10 min with bleaching buffer (1% H2O2 and 1% KOH).
Then, anodized aluminum substrate was washed with chromic acid and phosphoric acid mixture for 30 min to remove oxide layer.
The flue gas was washed with our acid aqueous media, and the sulfur is formed in the aqueous acid catalyst solution and was filtered out.
The trap was equilibrated with trifluoroacetic acid (0.1%, 300 μL), and the sample was loaded and washed with trifluoroacetic acid (0.1%, 300 μL).
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