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The interaction effect of cellulase (Cellic® CTec2), xylanase (Cellic® HTec2) and laccase along with a non-ionic surfactant (Tween® 80) on cellulose digestibility of unwashed and washed liquid ammonia pretreated energy cane bagasse was investigated.
Composition in hydrolyzate and washed liquid were analyzed using HPLC.
Steady-state fluorescence of washed liquid cultures expressing AmTrac-LE, -LS, -IS and -GS was acquired using a Fluoromax-P fluorescence spectrometer (Horiba Jobin Yvon, Kyoto, Japan) and 3.5 ml silica cuvettes (Hellma Analytics, Mullheim, Germany).
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The hydrolysate, washing liquid and thin stillage underwent AD.
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