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The basal chambers were washed and filled with DMEM without serum or fatty acids.
However, the knee of extremity No. 60 was washed and filled with Pregl's solution prior to closing the wound.
After 1 h of incubation, the mixtures were discarded and the wells washed and filled with 100 μl of the 3,3′,5,5′-tetramethylbenzidine 3,3′,5,5′-tetramethylbenzidine
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This water was used to wash and fill the drinking bottles as well as for preparation of tea from tea bags and to dissolve the granulated tea.
When the phantom needed to be prepared some time before use, a cork was taped into the open end of the drilled hole once it had been washed out and filled with water.
Get coconuts that have washed up and fill your inventory with everything you find.
People will get their car washed and a fill up at the same time.
The wells were washed 3 times with PBS containing 0.1% Tween 20 and incubated at room temperature with 100 µl of secondary antibody (PeproTech) diluted to 0.2 µg/ml, for 30 min. Each plate was washed 3 times and filled with 50 µl/well streptavidin-peroxidase (Roche Diagnostics; 1∶10,000 in PBS containing 0.1% Tween 20).
To collect bacteria attached to the plastic substratum, plates were washed 3 times and filled with 100 µl of fresh THY medium, sealed and floated on a sonicating water bath (Transonic 460/H ultrasonic bath) and sonicated for 3 sec at 35 KHz.
The plate was then washed with water and filled with 100 μl PBS.
Companionably, they set about weeding, washing gravestones and filling vases.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com