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The DMSO diffuses from a cell suspension stream into a neighboring wash stream flowing in parallel.
However, a greater cell density was achieved in most of the wash stream-containing fermentations relative to the control (YEP with no wash stream; Figure 3A).
The wash stream contains less than 2.5 g/L of monomeric glucose and xylose.
Fermentations by these three strains exhibited similar patterns; in that moderate levels of AFEX-CS wash stream improved cell growth but the degree of improvement decreased as the strength of the wash stream increased.
Potentially, the DA-based process can utilize the xylose-rich residue derived from pretreatment wash stream during fermentation.
While better xylose fermentation was achieved in KO11 than 424A LNH-ST) in AFEX-CS wash stream, the opposite trend was observed in dilute acid-CS wash stream due to the inhibitory nature of the wash stream and the strain robustness toward the inhibitors.
Similar(38)
Additionally, the wash streams containing the red-oils exiting the caustic section of an ethylene plant are usually rerouted to the QWT at a rate of few tons per hour.
The volumes of the wash streams were recorded.
In contrast, all tested AFEX-CS wastreamsams were high fermentable.
Distilled water was added to dilute the wash streams to 7.5% and 15.0% solid loading equivalent.
The grown cells were used to initiate fermentations Fermentations of E. coli KO11 and S. cerevisiae 424A LNH-ST) in wash streams of 7.5% and 15.0% solids-loading equivalent of the three types of pretreated CS were conducted in 2424A LNH-ST culture minroplates (BD Falcon #353047, San Jose, CA, USA).
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