Sentence examples for wash stages from inspiring English sources

Silica extractions were performed using QIAamp DNA micro extraction kits (Qiagen) following the manufacturer's guidelines, although using double the suggested volumes of all reagents prior to the wash stages (proteinase k, buffers AL and ATL, 100% Ethanol).

All incubation and wash stages were performed at 19°C.

Also, high dewatering levels leading to fewer wash stages reduces the chance of contamination in this highly manual operation.

High levels of compaction are of interest where greater extents of soluble contaminant removal are required to reduce number of wash stages and hence processing time and also where high cell densities (∼100 × 10 cells/mL) are required to mix with a matrix scaffold for tissue formation (Dar et al., 2002).

The hydrolysate wash stage has also proven to have a large role in maximizing ethanol yield.

This is followed by the wash stage where the basin is filled with filtered water to which CIDEZYME®-GI detergent is added.

The wash stage completed in the EVOTECH ECR eliminates tedious manual brushing of the endoscopes and results in cleaning that is safe, fast and consistent each time.

On the other hand, lysozyme and penicillin G were screened by addition at the wash stage in the wash and electroporation buffer, followed by incubation at 37°C for 30 minutes prior to electroporation.

Based on this research, the FDA concluded that manual cleaning of endoscopes is not required prior to processing in the EVOTECH ECR as long as a cycle including a wash stage is selected.

The mitochondrial pellet reformed, and after removing the supernatant, the pellet was added to 300 μl of resuspension buffer [human serum albumin, 0.5 mg/ml, 240 mM sucrose, 15 mM KH2PO4, 2 mM Mg(CH3COO 2 × 4 H2O, and 0.5 mM EDTA at pH 7.2] and was centrifuged at 15,000 g for 3 min as a further wash stage for the mitochondria.

After that, for separation of nanoparticles from each other, there were four washing stages by water and ethanol.