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Thus, high usage of wash solution may be mitigated.
Possible transport mechanisms for Pb under each wash solution are also discussed in this work.
The mixture was loaded to a NucleoSpin RNA Mini spin column and washed twice with DNA wash solution.
The abomasal content samples were diluted (1 1) by wash solution.
The wash solution was removed and two more washes were performed in 1 ml each of the guanidine/ethanol/glycerol wash solution.
Membranes were then washed with Church wash solution (1% SDS, 20 mM Na2HPO4, 1 mM EDTA).
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Cells were washed twice in BD Perm-Wash solution.
The cells were then washed twice with Perm/Wash solution and resuspended in staining buffer.
After two washes with Perm/Wash-solution (PharMingen, BD-Bioscience, Heidelberg, Germany) the slides were blocked for 30 minutes with 5% skimmed milk in Perm/Wash-solution.
Stop/Wash solution was then washed off in 3 10 minute PBT washes with agitation.
For antibody staining, cells were fixed with 4% PFA (20 min) and washed and resuspended in 1X perm/wash solution.
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