By Kenneth Phillips Britton, E. B. White, James Thurber, and Harold Ross The New Yorker, January 10 , 1931 P. 9In the middle of an exciting account of a storm in which the boat was being washed rapidly toward the cliffs below the Clew's house, Mrs. Miller asked.
The New Yorker, January 10 , 1931 P. 9In the middle of an exciting account of a storm in which the boat was being washed rapidly toward the cliffs below the Clew's house, Mrs. Miller asked.
After the removal of excess radioligands, cells were washed rapidly three times with ice-cold KRH.
After labeling, the samples were washed rapidly and transferred to a chamber of the superfusion apparatus (approximately 4 mg tissue in 0.2 mL volume) and superfused (0.2 mL/min) with medium gassed with 95% O2-5% CO2 at 37°C.
Drained pellets were redissolved in MLB/G containing activated thiopropyl agarose beads (GE Amersham, Piscataway, NJ) that had previously been washed rapidly in cold water per manufacturer's instructions, allowing 10 mg of dried beads per 35 mm well.
After preincubation, the samples were washed rapidly with the Krebs-Ringer and incubated for 15 min in 2.5 mL of this medium containing 5 µCi [3H]dopamine in an atmosphere of 95% O2-5% CO2 at 37°C with or without 6 µM GBR-12909 (dopamine reuptake inhibitor; Sigma-Aldrich, Zwijndrecht, the Netherlands).
Cultured cells were washed rapidly once with ice-cold PBS.
Immune complexes were collected by brief centrifugation and washed rapidly three times with IP buffer.
Cells were washed rapidly with ice-cold PBS, scraped, and collected.
Briefly, liposomes were loaded to pre-wetted filters (mixed cellulose ester, 0.45-μm pore size; Whatman) and washed rapidly with phosphate buffer.
To count exflagellation events either 10 µl purified gametocytes were resuspended in 50 µl GAM or 3 µl of tail blood from an infected mouse were washed rapidly in 1 ml GMB, pelleted and resuspended in 30 µl GAM.
