Decrease in LPS content in phage preparations was dependent on the washing intensity; intensive washing resulted in preparations of 11-40 EU/ml.
During the selection the stringency was gradually increased by decreasing the concentration of the target and library and enhancing the washing intensity.
Next, after 2 additional washes fluorescence intensity was measured.
After subsequent washes, fluorescent intensity in the sections was measured using NIH ImageJ.
Approximately one order of magnitude difference between results obtained in basic conditions of washing (100 to 400 EU/ml) and prolonged washing (10 to 40 EU/ml) indicates the strict relation between washing conditions or intensity and the level of purity of obtained preparations (Table 1).
A yearning for such moral intensity washes through the audiences of "Les Misérables".
And of course the various washes, and the intensity of bleach-line "whiskering" and the degree of destruction in the "destructed" styles.
After washing, the fluorescence intensity was determined by flow cytometry.
The cells were washed and fluorescence intensity determined by flow cytometry, as described.
Cells were washed and the intensity of fluorescence was assessed by flow cytometry using the CANTO II flow cytometer (BD Biosciences).
After washing, the fluorescence intensity of rhodamine-123 was recorded by confocal laser microscopy.
