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Tris-buffered saline was used as wash buffer.
The post hybridization wash was conducted using a Wash Buffer Kit (Roche NimbleGen, Inc .. Arrays were agitated consecutively in Wash Buffer I (2 minutes), Wash Buffer II (1 minute), and Wash Buffer III (15 seconds).
Following incubation, the wells were washed four times with 400 μl of provided wash buffer.
The His6-tagged protein was eluted with wash buffer containing 250 mM imidazole (pH 8.0).
The samples were then washed three times in wash buffer for 10 min at room temperature.
Tris wash buffer was purchased from BioRad (Hercules, CA).
To the pellet 5 ml wash buffer was added and swirled gently to resuspend DNA.
The cell pellet was washed with cell wash buffer for twice.
After that, the electrode was soaked in wash buffer for removing the non-hybridized target DNA.
Proteins were eluted in 5 cv of Wash Buffer II + 250 mmol/L imidazole.
Then the electrode was washed using wash buffer to remove the unimmobilized probe DNA.
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